DNA

Part:BBa_K2100028:Design

Designed by: Kathleen Brandes   Group: iGEM16_MIT   (2016-10-17)


pEXPR pERE5:eYFP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal PstI site found at 519
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal PstI site found at 519
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal PstI site found at 519
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 271
    Illegal EcoRI site found at 281
    Illegal PstI site found at 519
    Illegal AgeI site found at 302
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part expression vector was created by an LR reaction via gateway cloning. It is a promoter (flanked by L4, R1 sites) and a gene (flanked by L1, L2 sites) cloned into a backbone that has a negative selection marker between R4 and R2 sites. This part adheres to RFC 65 for recombination based cloning of mammalian parts.


Source

This comes from a synthetic promoter plus a jellyfish's genome.

References

Sathya G, Li W, Klinge CM, Anolik JH, Hilf R, Bambara RA. Effects of multiple estrogen responsive elements, their spacing, and location on estrogen response of reporter genes. Mol Endocrinol. 1997;11(13):1994–2003.